Blog Archives

Hot topics: Social network architecture of human immune cells unveiled by quantitative proteomics

The authors applied high-resolution mass-spectrometry-based proteomics to characterize 28 primary human hematopoietic cell populations in steady and activated states at a depth of >10,000 proteins in total. The protein copy numbers revealed a specialization of immune cells for ligand and

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Hot topics: Functional Selectivity in Cytokine Signaling Revealed Through a Pathogenic EPO Mutation.

Abstract: Cytokines are classically thought to stimulate downstream signaling pathways through monotonic activation of receptors. We describe a severe anemia resulting from a homozygous mutation (R150Q) in the cytokine erythropoietin (EPO). Surprisingly, the EPO R150Q mutant shows only a mild

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Hot topics: An atlas of human long non-coding RNAs with accurate 5′ ends

Using FANTOM5 cap analysis of gene expression (CAGE) data, the authors integrate multiple transcript collections to generate a comprehensive atlas of 27,919 human lncRNA genes with high-confidence 5′ ends and expression profiles across 1,829 samples from the major human primary

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Hot topics: The Ecstacy and Agony of Assay Interference Compounds

Editorial article in Biochemistry. [1] Alrich et al. (2017). Biochemistry 56(10):1363-1366. The Ecstacy and Agony of Assay Interference Compounds. [PMID: 28244742]

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Hot topics: Ligand and Target Discovery by Fragment-Based Screening in Human Cells

Description of a platform that marries fragment-based ligand discovery with quantitative chemical proteomics to map thousands of reversible small molecule-protein interactions directly in human cells, many of which can be site-specifically determined. [1] Parker et al. (2017).Cell. 168(3):527-541. Ligand and Target Discovery

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Hot Topics: Structural Basis of Substrate Recognition by the Multidrug Resistance Protein MRP1

Despite a flurry of mammalian ATP binding cassette (ABC) transporter structures in the last 2 years the Holy Grail has still been to determine how these diverse proteins interact with their transport substrates. Jue Chen and colleagues at the Rockefeller

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Hot Topics: The complete structure of an activated open sodium channel

Voltage-gated sodium channels (Navs) play essential roles in excitable tissues, with their activation and opening resulting in the initial phase of the action potential. The cycling of Navs through open, closed and inactivated states, and their closely choreographed relationships with

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